Test results of Maharishi Honey & Manuka Honey

1. Applied bacteria : Staphylococcus aureus IFO 12732

2. Preparation for test bacteria solution: Put bacteria on an general agar culture medium and leave 24 hours with 35 centigrade. Then dilute this solution with general bouillon and prepared.

3. Preparation for test materials. Dilute test materials with sterilized distilled water by 20, 50, 100, 200 times and prepared.

4. Test operation: Put 50ml of test solution into100ml flask and add 0.1ml of bacteria solution then keep with 35 centigrade.

5. Count number of bacteria. Count number of living bacteria in 1ml solution in flask by using standard agar. If the number is too high, use bouillon diluted by sterilized phosphoric acid relief solution.

CFU/ml--original number of bacteria : 4,800,000